Now I am fully convinced that the 5′ #RNA cap is reverse-transcribed. Show more
All the pieces of the puzzle fit together. An extra G is found at the 5′ end of cDNAs. The reverse-transcriptase can add extra nucleotides to blunt DNA/RNA duplexes. Addition of nucleotide monophosphates in the reaction enhances the addition of the complementary nucleotides to the cDNA. 5′ -5′ GpppG can hybridise to a CpC overhang of a RNA duplex. The cap probably can enter the active site in a similar manner. http://charles.plessy.org/tags/reverse_transcription/
I will be moving to #OIST (Okinawa Institute of Science and Technology) on August 1st, to join the Luscombe laboratory (Genomics and Regulatory Systems Unit). Expect more posts on marine creatures, although my core activities will still revolve around nucleic acids, genetic programs and free software.
The article also states: "Use of a software product incorporating such open source license requirements in a medical device may increase the risk that the medical device is disapproved by regulatory bodies due to being tamper-prone or otherwise unsafe."
Is that #FUD or did that happen at least once in the past ?
Bio-IT World published an article entitled "Open Source Pitfalls At The Biotechnology-High Technology Crossover" by Paul Liu (May 14, 2018) http://www.bio-itworld.com/2018/05/14/open-source-pitfalls-at-the-biotechnology-high-technology-crossover.aspx
In very brief, it reminds to people new to the field that open source softwares have licenses that vary in what they permit, and that one needs to be careful when combining software with different licenses. Key recommendations: monitoring, documentation, anticipation.
Major update of the CAGEr package for bioinformatics analysis of transcription start sites. Show more
Main improvements are memory efficiency, speed and parallelism, but I also added some new plot functions for quality controls.
The theme is "Open Science's Impact on Research Careers". https://euraxess.ec.europa.eu/worldwide/japan/european-research-day/european-research-nights-2018
Interesting... when replying to content-warning (CW) posts, the CW is sticky and has to be removed manually.
#Nanopore #protein #sequencing comes closer! Show more
In this week's issue of #PNAS, van Ginkel, ..., and Joo, from the Kavli Institute of Nanoscience, Delft University of Technology, Netherlands, demonstrate the use of FRET (energy transfer between fluorescent molecules that are very close each other) to detect key residues of a peptide passing through a pore (related to the AAA+ protease ClpXP from E. coli). https://doi.org/10.1073/pnas.1707207115
#Ultimaker printers are tested before being shipped. When preparing for the first print, we saw a drop of plastic extruded from the print core, so we thought that we did it right. But it was only leftovers from the maker's test. Actually, we had completely misunderstood the instructions, and did not even insert the material filament in the bowden tube! Now problem solved! Thanks fbrc8's video for opening our eyes. https://www.youtube.com/watch?v=uPWlZw8cTpc
Small interfering RNAs based on huntingtin trinucleotide repeats are highly toxic to cancer cells. Show more
Noticing that the gene causing Huntington's disease was also reducing the frequency of some cancers, Murmann, ..., and Peter (Northwestern University, Chicago) found that small RNAs containing the pathogenic sequences (triplet repeats) from the gene were toxic to cancer cells. These RNAs can be synthesized and therefore might become a new tool for medecine.
"Data notes" in "BMC Research Notes": a manuscript format sharing similar goals with the "Data descriptors" of "Scientific data".
The IMS applies interdisciplinary approaches which includes genome-wide medical science, immunology and physiology, bioinformatics, and molecular, biochemical and quantitative sciences.
Applicants must be distinguished international students in a Master's or Ph.D. course or young postdocs who received their Ph.D. within the last three years.
Open data has a source; this week's Policy Forum in Science reminds to respect it:
“researchers using open data should strive to conduct ethical studies relevant to local communities and return results to them”
Long version Show more
Hans Jonatan, born in the Caribbean in the 18th century to an enslaved African mother and European father, migrated to Iceland and founded a family there.
In the 21st century, researchers from Iceland studied the genes of 788 of his descendants (including whole-genome sequencing for 20 of them), and reconstructed 38% of his mother's genome. The data suggests that his mother was from the region spanned by Benin, Nigeria and Cameroon.
Rephrased from https://doi.org/10.1038/s41588-017-0031-6