Charles Plessy is a user on mastodon.technology. You can follow them or interact with them if you have an account anywhere in the fediverse. If you don't, you can sign up here.

Charles Plessy @charles_plessy@mastodon.technology

<Multi_key> <1> <1> <1> : "′" Show more

The edible ones are said to grow taller than 10 cm...

Earth Biogenome: an ambitious project to the of all living species on (actually, only eukaryotes; that excludes bacteria and archaebacteria, but includes unicellular organisms like yeast). Estimated data production in 10 years: 20 petabytes, well within the capacity of scientific infrastructures. earthbiogenome.org/

🍄Enjoyed the sight of in the central building's patio at .

Distance to nuclear speckeles measured by quantitative sequencing shows stronger transcription deeper in nucleus. Show more

Using Debian packages on CentOS with the Environment Modules system Show more

Lots of questions about abuse/moderation on Mastodon and how it’s different from Twitter.

This article goes into detail and explains a lot of how it works here.

Some things seem different (e.g. no global search, no ‘retweet’ with comments). But once you read the rationale behind it it makes sense.

*definitely* worth reading if you’re planning on sitting around here for a while.

blog.joinmastodon.org/2018/07/

A yeast cell witn a single chromosome was created by Chinese researchers. Show more

Artificial DNA sequences used to study the shape of chromosomes. Show more

The scientific journal Molecular Systems Biology is introducing "Structured Methods for reproducible science", putting more effort on conveying useful reagent lists, and allowing writers to publish extensive bullet-point methods instead of the condensed narratives. Reformatting methods from bullet to condensed style was and still is a source of errors and typos. msb.embopress.org/content/14/7

Adiconis and co. published an analysis in Nature Methods showing that CAGE is the best method for 5'-end RNA-. It is a nice benchmark with overall a strong design.

doi.org/10.1038/s41592-018-001

Our method was also tested and performed poorly in the hands of the authors. However, they used 7.5 μg of total as starting material, which is totally out of the range that we designed nanoCAGE for. Therefore, I have posted a response on explaining that fact.

pubpeer.com/publications/CA8FC

When BLT is about beads and transposases and not bacon and tomatoes Show more

Needing a new laptop at work that can run Office, I was tempted to try again after more than a decade on OSX, because I am quite curious about the windows for Linux. Unfortunately, the laptop that I wanted to buy was only available in Japanese, and despite we are in 2018 it seems that MS still sells "single-language pack" systems were English can not be installed. Thus, I am going for a Mac instead. And of course running on my main desktop. Sorry MS, you must do better!

Poster flash talks in conferences: nice idea, but it takes precious time, so I was unsure if it was worth doing it, but recently I realised that it is an excellent opportunity to show videos, which obviously can not be on the posters (at least for the moment...).

Ranting on about authors who did not seem to check that their introduction was still up to date before submitting their paper: pubpeer.com/publications/4EDEA

High-throughput homologous recombination mutagenesis in yeast with libraries of plasmids providing the DNA template for recombination and expressing a guide RNA for inducing the recombination with a CRISPR-Cas9 break: 3 papers in last week's issue of Nature Biotechnology. nature.com/nbt/volumes/36/issu

This year's application call for faculty positions at (Okinawa Institute of Science and Technology) is out ! Deadline: August 31st. groups.oist.jp/facultyposition

Transparency on scientific instruments Show more

"Preproducibility", a new word to foster reproducible research. Show more