Now I am fully convinced that the 5′ #RNA cap is reverse-transcribed. Show more
All the pieces of the puzzle fit together. An extra G is found at the 5′ end of cDNAs. The reverse-transcriptase can add extra nucleotides to blunt DNA/RNA duplexes. Addition of nucleotide monophosphates in the reaction enhances the addition of the complementary nucleotides to the cDNA. 5′ -5′ GpppG can hybridise to a CpC overhang of a RNA duplex. The cap probably can enter the active site in a similar manner. http://charles.plessy.org/tags/reverse_transcription/
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