We finished to assemble the of the local population of the dioica near on the island. We used sequencers and it allowed to assemble whole chromosomes into single contigs. Our preprint is available in : biorxiv.org/content/10.1101/20 Our results open the way to interesting comparisons with related populations, to better understand species in the .

20 years after reading the famous "Maniatis" book on molecular cloning, I read an actual article from him for the first time. 

The article demonstrates trans-splicing of nematode and trypanosome splice leaders in mammalian cells. (doi.org/10.1038/360692a0). "Trans-splicing" is the molecular basis for the gene structure in eukaryotic cells.

Receiving archives from your favourite funding agency with filenames encoded in Shift_JIS? Thanks to "Ask Ubuntu" I found out that "The Unarchiver" theunarchiver.com/ can convert them on the fly. askubuntu.com/questions/935022

Centromere-specific antibody-mediated karyotyping of Okinawan Oikopleura dioica suggests the presence of three chromosomes. 

Our latest work is now available before peer-review on . is the animal that we are studying at . Its is tiny and therefore its chromosomes are difficult to visualise. For that reason, we needed to make these experiments to confirm their number.

The Okinawa Institute of Science and Technology Graduate University hires to fight #COVID and develop #DNA sequencing technologies ! 

's DNA section hires one Testing and Sequencing Specialist and one R&D and Sequencing Specialist. is a very nice place to live, and has been relatively safe from covid with no cases for a month. And OISTs facilities are, in one word, fantastic.

oist.jp/careers/testing-and-se

oist.jp/careers/rd-and-sequenc

Mankind is still losing genes of olfaction. 

In today: “Common homozygosity for predicted loss-of-function variants reveals both redundant and advantageous effects of dispensable human genes”, from Rausell, collaborators and Abel, of the hospital and other research institutions, shows that among 166 genes that appear to be often lost or non-functional in people's genomes, 41 are receptors. Quel dommage. pnas.org/content/117/24/13626

have a specific gene family, called "Ya" by its discoverers, which they inject as in their host plant in a way that increases their performance. Published this week in by Chen, Singh, and their colleagues from the Department of , John Innes Centre, , UK. pnas.org/content/117/23/12763

Impressive laser sheet imaging and 3D modelling of giant Bathochordaeus tunicates living in the deep . Here is a link to the news article at the Bay Aquarium Research Institute mbari.org/deep-piv-3d-flow/

Oxford Technologies is releasing "LamPORE", an assay for sequencing the SARS coronavirus 2. The "LamP" in the name means that the method is "isothermal", which in practice means that only a heat block is needed to process the sample instead of an expensive PCR machine. This is one more step forward having virus detection at the "point of care" instead of in laboratories. Even before -19, the industry has been working hard for this in the past decades (for flu, etc.) nanoporetech.com/covid-19/lamp

this article includes a nice informal comparison of #PEPPPT v. #ROBERT approaches for #covid19 tracing apps « France’s Inria and Germany’s Fraunhofer detail their ROBERT contact-tracing protocol » tcrn.ch/2VlI7ja via @techcrunch

Kudos to Li and coll. from the Chinese Academy of Sciences, who submitted to peer review on November 6 2019 an article with a method to reduce the cost of sequencing in bats, where they wrote "Active surveillance is both urgent and essential to predict and mitigate the emergence of bat-origin CoV in humans and livestock." msphere.asm.org/content/5/1/e0

A slipped-CAG DNA-binding small molecule induces trinucleotide-repeat contractions in vivo 

This is an article published this week by Nakamori, Panigrahi and their collaborators in Nature Genetics, where they show a small drug designed to force (CAG)n repeats to form stable hairpins, which causes these repeats to reduce their size. This is important because expansion of these repeats is part of the mechanism causing Huntington's disease. nature.com/articles/s41588-019

Tagmentation of DNA/RNA hybrids! 

In today: researchers from Beijing and their collaborators have managed to use the Tn5 transposase to "tagment" /#RNA hybrids (pnas.org/content/117/6/2886). When we tried in my previous laboratory, we either could not make it work or did not realise it works, because as seen in the paper's supplemental figures 9 and 10, the difference of the size profile before and after reaction is easy to miss (compared with DNA/DNA tagmentation)...

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